Title: Antitumor Activity of Nodosin on Rat C6 Glioma Cells
Abstract: Objective To evaluate the antitumor effect of nodosin on rat C6 glioma cells.Methods Morphologic observation of nodosin on C6 cells was performed under phase contrast microscope,cell viability was tested by MTT assay,and cell proliferation was detected by BrdU assay.The cytotoxicity of nodosin was evaluated by lactate dehydrogenase(LDH) leakage method and Hoechst 33342 staining was used to test the cell apoptosis.Results Nodosin could inhibit the cell viability of the rat C6 glioma cells in time-dependent and dose-dependent manner at hour 12 and 24,and it had no cytotoxicity on C6 glioma cells within 20 μmol·L-1.The cell proliferation rate was reduced to 74.6 % after treatment with 20 μmol·L-1 of nodosin at hour 24,and nodosin promoted the apoptosis of C6 cells.Conclusion Nodosin has antitumor action on C6 cells by inhibiting cell proliferation and inducing apoptosis.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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