Title: Effect of Berberine Derivate B-119 on Inhibition of Tumor and Vascular Endothelial Cell Proliferation
Abstract:Objective To investigate the effect of berberine derivate B-119 against the proliferation of tumor cells and against the proliferation,migration and tube-like structure formation of vascular endotheli...Objective To investigate the effect of berberine derivate B-119 against the proliferation of tumor cells and against the proliferation,migration and tube-like structure formation of vascular endothelial cells.Methods MTT assay was applied to screen the anti-proliferative effect of B-119 on 8 kinds of tumor cell and ECV304 cells in vitro.The impact of B-119 on the migration of ECV304 cells was measured by scarification test,and the impact of B-119 on tube-like structure formation of ECV304 cells was examined under microscope.Results B-119 had different degrees of inhibition to these 8 tumor cell lines,and the IC50 of B-119 to these tumor cells were 0.76~26.83 mg/L.B-119 could inhibit the proliferation of ECV304 cells in a concentration-dependent and time-dependent manner.After being treated with B-119 for 24 h,48 h and 72 h,MTT assay showed the IC50 of B-119 to ECV304 were 59.22,12.08 and 3.31 mg/L respectively.The migration of ECV304 was reduced,and the inhibition effect of B-119 on ECV304 cells was strengthened with the increase of concentrations.After being treated with B-119 at the concentrations of 1.25,2.5 and 5 mg/L for 24 h,the number of ECV304 cells migration was gradually decreased and the migration inhibition ratios were 49.29%,59.71% and 71.94% respectively(P0.01).B-119 could inhibit the tube formation of ECV304 cells,After being treated with B-119 at the concentrations of 10,20 and 30 mg/L for 18 h,the tube formation inhibition ratios were 35.71%,57.14% and 67.86% respectively(P0.01).Conclusions B-119 could inhibit the proliferation of 8 kinds of tumor cell and also inhibit the proliferation,migration and tube-like structure formation of ECV304 cells.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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