Title: Genetic diversity in Avena based on AFLP analysis
Abstract:Genetic diversity of 34 Avena sativa varieties and 8 A.nuda varieties from China,Canada,Australia,Europe and other countries was studied using amplified fragment length polymorphism(AFLP).Five primer ...Genetic diversity of 34 Avena sativa varieties and 8 A.nuda varieties from China,Canada,Australia,Europe and other countries was studied using amplified fragment length polymorphism(AFLP).Five primer pairs with higher polymorphic were selected from 30 EcoRI/MseI primers,268 AFLP markers were generated and 185 were polymorphic(69.0%).The highest polymorphic rate was 78.6% with primer E-AGG/M-CTA.In Nei's index calculation,the average variation coefficient was 0.166 4 and Shannon diversity index was 0.220 6.All cultivars Pairwise similarity coefficient varied from 0.488 1 to 0.988 0,with 0.012 0-0.717 2 genetic distance.The fingerprinting data were further analyzed using UPGMA methods which clustered the 42 varieties into six main distinct groups at 0.748 genetic similarity coefficients.A.sativa and A.nuda were divided into two groups at 0.59,which was in accordance with traditional classification.The genetic relationship revealed by AFLP was consistent with original sources;genetic distance was closely associated with geographical distributions.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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