Title: Expression of OCTN2in human epididymis and its significance
Abstract: Objective:To study the expression of OCTN2mRNA and protein in human epididymis so as to provide a further theoretical basis for the mechanism of L-Carnitine transport.Method:We collected epididymides from aprostate cancer patient and determined the expressions of OCTN2mRNA and it's protein in the caput,corpus and cauda of the epididymis by RT-PCR and Westernblot and calculated the relative content of the protein.Result:OCTN2Mrna was expressed in the caput,corpus and cauda of the epididymis and the expression level in the corpus and cauda of the epididymis were higher than in the caput.OCTN2protein was also expressed in human epididymis and the relative content was(0.71±0.09)in the caput of the epididymis.It was lower than the relative content(0.95±0.22)and(0.99±0.15)respectively in the corpus and cauda of the epididymis.Conclusion:The expression of OCTN2mRNA and protein in human epididymis was certificated by RT-PCR and westernblot.They both indicated that the expression level was low in the caput of the epididymis and the expression level was high in the corpus and cauda of the epididymis.This conclusion will play an important role in the study of the mechanism of L-Carnitine transport.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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