Title: Influence of autophagic apoptosis induced by curcumin on human gastric carcinoma SGC7901 cells
Abstract: 【Objective】 To investigate the molecular mechanisms of autophagic apoptosis induced by curcumin in human gastric cancer cell SGC7901 in vivo.【Methods】 The human gastric carcinoma SGC7901 cells were cultured in vivo containing different concentrations of curcumin.Cell viability was analyzed by MTT assay.MDC staining and Hoechst 33342 were used to examine autophagy and apoptosis induced by curcumin.The apoptosis rates were analyzed by flow cytometry.The expression of NF-κB,LC3,and Beclin1 was examined by Western blotting.【Results】 The proliferation of SGC7901 cells was significantly inhibited by curcumin in a dose-dependent manner.After 40 μmol/L of curcumin treatment for 12h and 24 h,MDC and Hoechst 33342 staining were performed,and the autophagy in SGC7901 cells was increased compared to the control group in 12 h but the autophagy was decreased in 24 h.Then we performed Hoechst 33342 staining after 40μmol/L of curcumin treatment under the same conditions.The results showed that autophagosomes were increased in a time-dependent manner compared to the control group and the results of FCM indicated that the apoptosis rate was increased significantly after 40μmol/L of curcumin treatment for 24 h.In the course of autophagic apoptosis induced by curcumin,the autophagic stage was prior to executional stage of apoptosis.Western blot analysis showed that the expression of apoptosis related protein NF-κB,autophagy related proteins LC3 and Beclin1 was all up-regulated.【Conclusion】 Curcumin could significantly suppress the proliferation of SGC7901 cells through induction of autophagic apoptosis and the up-regulation expression of NF-κB,LC3 and Beclin1protein might be involved in the curcumin-induced autophagic apoptosis.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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Cited By Count: 1
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