Title: Extraction,Amplification and Sequence Analysis of Xiajiadian Ancient Human Bone DNA
Abstract: Ancient DNA was extracted from human bone remains (varying in age from about 2 000 to 5 000 years old) recovered from the archaeological sites.Two mitochondrial DNA fragments from 11 210 to 11 414(205 bp) and from 8 196 to 8 316 (121 bp) were amplified from the extracts using the polymerase chain reaction (PCR) and sequenced directly.Evidence is presented that the amplified sequences are authentic and do not represent contamination by extraneous DNA.The result shows that 11 248,11 249,11 283 and 11 293 are highly variable sites and ⅢM11 and ⅢM12 have high homogeneity, but M89 and AM4 have lower homogeneity compared with ⅢM11 and ⅢM12.there are remnents of genetic information in the ancient bone DNA,which have important implication for anthropology,archaeology and molecular evolution.
Publication Year: 2001
Publication Date: 2001-01-01
Language: en
Type: article
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