Title: Determination of Costunolide and Dehydrocostuslactone in ShuGan HeWei Granules by HPLC
Abstract: Objective: To establish the method for determining the contents of costunolide and dehydrocostuslactone contained in ShuGan HeWei granules by HPLC. Methods: Chromatographic column: C18column(250 mm×4.6 mm, 5 μm), mobile phase: methanol-water(65:35); flow rate: 1.0mL/min; detection wavelength:225 nm. Results: Costunolide showed good linear relationship in the range between 0.073 8 and 0.492 μg, r=0.999 7, average recovery rate was 99.75%, RSD=0.88%; dehydrocostuslactone demonstrated good linear relationship in the range between 0.078 6 and 0.524 μg, r=0.999 8, average recovery rate was 99.58%, RSD=0.48%. Conclusion: The results of the tests for six batches of samples show that the method, simple and accurate, could be used for quality control of ShuGan HeWei granules.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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