Title: Cloning,Sequencing and Expression Profile Analysis of A Polygalacturonase-inhibiting Protein (PGIP) Gene in Cotton
Abstract:Plant polygalacturonase-inhibiting proteins(PGIPs)play an important role in the defense system by effectively and specifically binding to and inhibiting the endopolygalacturonases secreted by pathogen...Plant polygalacturonase-inhibiting proteins(PGIPs)play an important role in the defense system by effectively and specifically binding to and inhibiting the endopolygalacturonases secreted by pathogen during its invasion.In this study,a gene encoding the PGIP of cotton(Gossypium hirsutum),named GhPGIP1,was cloned and sequenced,which had 993bp in length and encoded a peptide of 330 amino acids with a molecular weight of 37.0 kDa and pI of 8.30.Compared with the corresponding genes from other 9 species,the nucleotide and amino acid homo-logies were 65.6%-70.3% and 63.4%-68.6%,respectively.The deduced protein sequence had 6 potential N-glycosylation sites and 10 cysteine residues in N-and C-terminal.The sequence alignment of deduced amino acid of GhPGIP1 with those of other 9 species indicated that there were 5 conservative leucine rich repeat(LRR)domains,the typical character of proteins with defense ability and most of the Leucines being highly conserved.Semi-quantitative RT-PCR disclosed that GhPGIP1 were constitutively expressed in root,stem and leaf,and the expressed amount in leaf was the most.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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Cited By Count: 2
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