Title: Effects of testosterone and androgen blocker bicalutamide on the cell growth of androgen-dependent prostate cancer cell line LNcap-FCG
Abstract:Objective To evaluate the effects of testosterone and androgen blocker bicalutamide on the growth of androgen-dependent prostate cancer cell line LNcap-FCG in vitro. Methods Androgen-dependent prostat...Objective To evaluate the effects of testosterone and androgen blocker bicalutamide on the growth of androgen-dependent prostate cancer cell line LNcap-FCG in vitro. Methods Androgen-dependent prostate cancer cell line LNcap-FCG were cultured in vitro and testosterone was added to the medium at different concentrations of 10-10, 10-9, 10-8, 10-7, 10-6, 10-5 mol/L. The mixtures of 10, 50μmol/L bicalutamide with different concentrations of testosterone were also added to the medium in another group cells. The 5-bromodeoxyuridine(BrDU) incorporation method was used to measure the status of cell proliferation. Results At seventy-two hours after adding testosterone or the mixture of testosterone and bicalutamide, the BrDU absorbance (A )value of the control group LNcap-FCG was 1.2411. The lower concentration (10-10, 10-9 , 10-8, 10-7 mol/L) testosterone promoted the growth of LNcap-FCG and the corresponding A value increased to 1. 4247, 1.5463, 1.5110, 1.4609, respectively. While higher concentrations ( 10-6, 10-5 mol/L) of testosterone restrained the growth of the cells and the corresponding A value decreased to 1. 1239, 0. 9967 respectively. The inhibition effect of bicalutamide was enhanced with the increasing of concentrations. All groups showed significant difference compared with the controls. Conclusions Testosterone has bidirectional action on the cell proliferation according to the different concentrations. Bicalutamide inhibits the proliferation of LNcap-FCG cell line in a dose-dependent manner in vitro.Read More
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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