Title: [Dynamic analysis of HBV cccDNA in HepG2 cells infected with Ad-1.2 HBV].
Abstract: o study the replication of hepatitis B virus (HBV) in HepG2 cells infected with Ad-1.2 HBV.HepG2 cells were transfected with adenovirus containing 1.2 copies of HBV DNA. The expression of HBV antigens were detected in the culture medium by means of enzyme-linked immunosorbent assay (ELISA), and the covalently closed circular DNA (cccDNA) in the cells was extracted with plasmid extraction kit and detected by real-time PCR with selective primer after treatment with mung bean nuclease.HBsAg, HBeAg and HBV cccDNA were all detected in HepG2 cells after tranfection with Ad-1.2 HBV. HBV cccDNA was detected 1 day after the infection, reaching the peak level 4 days after infection.Ad-1.2 HBV-infected cells can serve as the model for screening and evaluation of antiviral agents.
Publication Year: 2007
Publication Date: 2007-09-01
Language: en
Type: article
Indexed In: ['pubmed']
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