Title: Preliminary Isolation and Identification of Nephropathogenic Stram of Avian Infectious Bronchitis Virus
Abstract: A strain of Nephropathogenic avian Infectious bronchitis Virus was isolated from died chickens that they were suspected to be infected by avian infectious bronchitis from a broiler flocks farm in Harbin city.Biological characterizations of the virus were investigated by dwarfing embryonated chicken eggs,haemagglutination test and electron microscope morphological observation,NDV interference test,virus pathogenicity to healthy chickens and RT-PCR of N gene.Results showed that the infected embryos of F4 began to die or form dwarf embryo;and that Virus could not agglutinate chicken's red blood cells;and that the virus particle was approximately 80 to 120 nm in diameter,with typical morphologyof Coronavirus;and that the replication of NDV LaSota strain was affected in chicken embryo infected by the strain;and that a 438-bp DNA fragment from the N gene was amplified by RT-PCR.The virus isolated was primarility identificated as infectious bronchitis virus.in addition,six days of chickens were inoculated with the F4,post inoculation 7 days,some of chickens began to die,the mortality rate as high as percentage 67(6/9),The kidney of the death chickens is swell and mottle prominently,with typical pathological changes of infectious bronchitis.All of these demonstated that the stain isolated from broiler fowls was nephropathogenic infectious bronchitis virus
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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