Title: Optimization of fermentation medium for antimicrobial activity production by endophytic strain EJH1 from Honeysuckle with response surface methodology
Abstract: Response surface methodology was used to optimize the submerged fermentation medium of antimicrobial activity production of endophytic strain EJH1 from Honeysuckle.In the fist optimization step,a Plackett-Burman design was undertaken to screen the key factors rapidly from the 9 related factors.By analyzing the statistical regression,the sodium glutamate and KH_2PO_4 were found to be the most important factors for the production of antimicrobial activity.Based on the results of our previous Plackett-Burman design,a five-level two-factor Central Composite design was applied to optimize the production of antimicrobial activity.The critical factors selected for the investigation were mentioned above.By analysis of the 3D plots and their corresponding plots,the optimum values of the concentrations of sodium glutamate and KH_2PO_4 for obtaining the most production of antimicrobial activity were 5.12 g·L~-1 and 2.13 g·L~-1,respectively.The diameter of antimicrobial circle increased from 18.21 mm to 20.27 mm in optimized medium condition.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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