Title: Changes of P15 gene promoter region methylation following decitabine treatment in the plasma of patients with myelodysplastic syndromes
Abstract:Objective:To detect the methylation status of P15 gene promoter region in the plasma of patients with myelodysplastic syndrome(MDS),and to investigate the demethylating effects of dicitabine.Method:Me...Objective:To detect the methylation status of P15 gene promoter region in the plasma of patients with myelodysplastic syndrome(MDS),and to investigate the demethylating effects of dicitabine.Method:Methylation-specific PCR(MSP) was used to detect the methylation status of P15 gene promoter region in the plasma of 4 patients,among them,one newly diagnosed MDS,three progressed into acute leukemia,with myelodysplastic syndrome before and after treated with decitabine plus semis CAG therapy.Result:Four cases were found to have an increased methylation in the promoter region,after treated with decitabine plus semis CAG,3 cases were not found increased methylation.In 4 cases,2 cases gain clinical response,and the other 2 cases were useless.Conclusion:P15 gene hypermethylation is associated with MDS pathogenesis.Decitabine has demethylating effect on the plasma from MDS patients The methylation status of P15 gene may serve as an important molecular marker to provide evidence for MDS diagnosis and predict the prognosis.Read More
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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