Title: [Affinity maturation of a single-chain antibody against hepatocellular carcinoma].
Abstract: OBJECTIVES To obtain a single-chain antibody with high affinity against hepatocellular carcinoma (HCC). METHODS A second single-chain antibody mutant library was established using an error-prone PCR and a phage display. Single-chain antibodies with high affinity for hepatocellular carcinoma were selected using ELISA. RESULTS The content of the second single-chain antibody mutant library was about 4.5 x 10(7). Two selected mutants, M90 and M116, were obtained after 3 rounds of panning and ELISA. Immunoassay showed that both M90 and M116 could bind to human HCC cells. The relative affinity of M90 was 1.7-fold higher than that of the original antibody, and M116 was 2-fold higher than that of the original antibody. CONCLUSION Error-prone PCR is an effective and simple method for affinity maturation of antibodies isolated from a phage antibody library.
Publication Year: 2006
Publication Date: 2006-03-01
Language: en
Type: article
Indexed In: ['pubmed']
Access and Citation
Cited By Count: 2
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