Title: Clinical Significance of Perinuclear Antineutrophil Cytoplasmic Antibodies and Anti-Saccharomyces cerevisiae Antibodies in Inflammatory Bowel Disease
Abstract: Background:Perinuclear antineutrophil cytoplasmic antibodies(pANCA) and anti-Saccharomyces cerevisiae antibodies(ASCA) are immunoglobulins closely related with inflammatory bowel disease(IBD).The role of pANCA and ASCA in the diagnosis of ulcerative colitis(UC) and Crohn's disease(CD) remains further verification.Aims:To investigate the significance of pANCA and ASCA in the diagnosis and differential diagnosis of UC and CD.Methods: Serum samples were obtained from 64 patients with UC,62 patients with CD and 56 healthy controls.Levels and positivity rates of pANCA and ASCA were determined by enzyme-linked immunosorbent assay(ELISA).The sensitivity,specificity, positive predictive value(PPV) and negative predictive value(NPV) of pANCA,ASCA and their combination were analyzed.Results:Serum pANCA level was significantly higher in UC group than that in CD group and healthy controls [(584.22±347.70) pg/ml vs.(304.99±211.10) pg/ml and(390.92±82.82) pg/ml,P0.01],so was the positivity rate of pANCA(50.0%vs.14.5%and 14.3%,P0.01).The level and positivity rate of serum ASCA among these three groups had no significant difference.The sensitivity,specificity,PPV and NPV of pANCA~+and pANCA~+/ASCA~-in diagnosis of UC were 50.0%,85.7%,80.0%,60.0%and 42.2%,89.3%,81.8%,57.5%,respectively,and those of ASCA~+and ASCA~+/ pANCA~-in diagnosis of CD were 8.1%,87.5%,41.7%,46.2%and 3.2%,91.1%,28.6%,45.9%,respectively. Conclusions:Positive pANCA favors the diagnosis of UC.Detection of pANCA together with ASCA is helpful for the differential diagnosis of UC,but is not of high value for the diagnosis of CD.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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