Title: Protective mechanism of cerebral ischemic preconditioning combined with sodium ferulate on global ischemia reperfusion injury
Abstract: Objective To explore the protective effects and the mechanism of cerebral ischemic preconditioning combined with sodium ferulate (SF) on global ischemia (I) reperufusion injury of rats.Methods Global cerebral ischemia was induced in rats by four vessel occlusion.Animals were allocated to the following groups before the experiment:sham (S);IP;SF+IP;and I.Nissl staining and terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick end labeling (TUNEL) were used to count the number of alive neurons and apoptotic cells in the cortex and hippocampal CA1 of rats.Immunohistochemistry with Fas protein polyclonal antibody and Caspase 8 protein polyclonal antibody were used to explore the changes in the Fas protein and Caspase 8 protein expression after global cerebral ischemia.Results The number of apoptotic cells in SF+IP group was less significantly than that in IP group and I group.The alive neurons in cortex and hippocampus in SF+IP group showed no significant changes,but those in I group were reduced remarkably after 7 days of global cerebral ischemia.The cells positive for Fas protein expression in SF+IP group were less than those in I group significantly,and the Caspase 8 protein expression cells in SF+IP group were less significantly than those in IP group and I group.Conclusion Ischemia preconditioning combined with sodium ferulate can enhance the effects of cerebral ischemic preconditioning on global ischemia reperufusion injury,possibly by reducing the expression of the expression of Caspase 8 and subsequently suppressing the apoptosis in cortex and hippocampal CA1 region induced by global ischemia.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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