Title: Effect of Modified Shenfu Decoction on Apoptosis-related Factors Caspase-3 and Bcl-2 Expression in Pulmonary Fibrosis Rats with Kidney-yang Deficiency
Abstract: Objective: To observe the effect of modified Shenfu Decoction on the pulmonary fibrosis pathological changes and caspase-3 and Bcl-2 expression levels in the pulmonary fibrosis rats of kidneyyang deficiency,and to explore the possible mechanism. Methods :Sixty Wistar rats were randomly divided into normal group,model group and intervention group. Kidney-yang deficiency of pulmonary fibrosis was induced in the rats of the model group and intervention group,and then the intervention group was treated by Shenfu Decoction. After treatment,the rats of three groups were executed,and the lung was made into routine paraffin section. HE staining was performed for the observation of alveolitis and pulmonary fibrosis,and the expression of caspase-3 and Bcl-2 protein in rat lung tissue was detected by immunohistochemical method. Results : Obvious expression of caspase-3 and Bcl-2 was presented mainly in alveolar epithelial cells,pulmonary macrophages and infiltrated inflammatory cells. On day 28, model group and intervention group had higher expression of caspase-3 and Bcl-2 protein than that in the normal group(P 0.05);on day 42,caspase-3 expression in rats of intervention group was significantly lower than the model group(P 0.05), and the expression of Bcl-2 in the intervention group was enhanced, higher than that of the model group(P 0.05). Conclusion:During the development of pulmonary fibrosis in kidney yang deficiency rat,the apoptosis of lung tissue cells play an important role,and modi-fied Shenfu Decoction can slow down the progress by regulating caspase-3 and Bcl-2 protein expression to reduce apoptosis.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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