Title: Determination of rutin and quercetin in Prunella vulgaris by HPLC
Abstract: OBJECTIVE To establish an HPLC method for determination of rutin and quercetin in Prunella vulgaris.METHODS The samples were separated on the Alltima C18( 4.6 mm × 250 mm,5 μm) column which was eluted with methanol and water ( 0.1 % acetic acid) with detective wavelength at 350 nm and flow rate at 1.0 mL·min -1,column temperature at 30 ℃.RESULTS The results indicated that the two compounds were basically isolated.The linear ranges of rutin and quercetin were 0.0149-0.2380 mg· mL -1( r = 0.9999) ,0.0019-0.0306 mg·mL -1 ( r = 0.9998) respectively.The average recoveries were 97.78% ( RSD = 0.83% ) , 101.18% ( RSD = 0.83% ) respectively.The contents of rutin in Prunella vulgaris from Anhui,Hubei,Guizhou,Jiangxi,Guangxi were 0.3527,0,0.2337,2.1164,0.9578 mg·g -1 crude drug respectively,and the contents of quercetin were 0.0872,0.0473,0.0721, 0.5219,0.1755 mg·g -1 crude drug respectively.CONCLUSION The method is sensitive,accurate,and can be used to control the quality of Prunella vulgaris.;Determination.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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