Title: Effect of different dosage of Yiqi Huayu Huatan Yangyin Formula Decoction on liver structure and expression of PPARγ in rats with liver fibrosis
Abstract: Objective To study the effect of differentdosage of Yiqi Huayu Huatan Yangyin Formula Decoction on liver structure and expression of peroxisome proliferator-activated receptor γ( PPARγ) in rats with liver fibrosis. Methods 110 male healthy SD rats were randomly allocated into control( n = 10),liver fibrosis( n = 20),colchicine( n = 20),YHHY-low-dosage-treated( n = 20),YHHY-middle-dosage- treated( n = 20),YHHY-high-dosage-treated( n = 20) groups. Rats in control group were fed by normal food and water,while the others were prepared of liver fibrosis by the methods ofsubcutaneous injection of carbon tetrachloride( CCl4) + low-protein and high-fat diet + liquor. After 12 weeks of treatment,liver tissue histological examination was carried out to examine the liver pathology. The expression levels of PPARγ mRNA and protein were measured by RT-PCR and Western blot. Results In contrast with control group,significant fibrosis and PPARγ mRNA and protein expressions of the liver tissue were decreased significantly on liver fibrosis group( P0. 05). Compared with liver fibrosis group,liver fibrosis was inhibited in colchicine,YHHY-middle-dosage- treated,YHHY-high-dosage-treated groups( P 0. 05).However,no statistically significant differences were found between YHHY-middle-dosage- treated and YHHY-high-dosage-treated groups( P0.05). Conclusions Middle-dosage or high-dosage of YHHY could attenuate liver fibrogenesis in rats. Its effective mechanism might be related to increase PPARγ mRNA and protein expressions in liver tissue.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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