Title: Inhibition effect of resveratrol on proliferation of lung cancer cells H460 by up-regulating expression of miR-622
Abstract:Subject To investigate the inhibition effect of resveratrol on proliferation of lung cancer cell H460 by upregulating the expression of miR-622. Methods H460 cells were treated with resveratrol with d...Subject To investigate the inhibition effect of resveratrol on proliferation of lung cancer cell H460 by upregulating the expression of miR-622. Methods H460 cells were treated with resveratrol with different concentrations. CCK-8 assay was used for checking the proliferation in the H460 cells. The miRNA taqman assay was employed to analyze the expression of miR-622. The oligos miR-622 simulant, miR-622 inhibitor and their control were transfected into in H460 cells with aid of Lipofectamine 2000. Results The proliferation rate of H460 cells 48 hours after treated with 50 μmol/L resveratrol was significantly dropped to(49.77±1.33)%(p0.01). The miR-622 was highly expressed by(13.48±2.16) folds in cells 48 hours after treated with resveratrol(p0.01). Expression level of miR- 622 could be significantly enhanced after transfection of miR-622 simulant and the proliferation rate of H460 cells would significantly reduced to(60.27±5.93)%( p0.01). Transfection of miR-622 inhibitor could reduce the expression of miR-622 and the proliferation rate of H460 cells in resveratrol treated group would increase to(86.67±1.11)%,significantly higher than the miR- 622 inhibitor control group(51.67%±4.22%),showing significant difference( p0.01). Conclusion Resveratrol can inhibit the proliferation of H460lung cancer cells and the inhibition effect might be closely related to up-regulation of expression of miR-622.Read More
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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