Title: DETERMINATION OF HUMAN HEPATIC CYTOCHROMES P450 AND ACTIVITY OF CYP1A1 AND CYP2A6
Abstract: Microsomes were isolated from adult human livers. Protein and cytochrome P450 content in microsomes and the activities of its isoenzymes, ethoxyresorufin O dealkylase(CYP1A1) and coumarin 7 hydroxylase(CYP2A6), were determined. The results showed that no significant effect of store at -80°C for six months on the activity of human liver microsomes. The methods utilized to determined the activity of CYP1A1 and CYP2A6 were simple, stable and repeatable.
Publication Year: 1999
Publication Date: 1999-01-01
Language: en
Type: article
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