Title: Study on the effects of Ginkgo biloba extract on the antioxidation ability and the serum TNF-α contents in adjuvant arthritis rats
Abstract: OBJECTIVE To investigate the antioxidation ability of Ginkgo biloba extract(EGb761) on adjuvant arthritis(AA) in rats and to probe into its underlying mechanism.METHODS The AA was induced by Freund's complete adjuvant(FCA) in male SD rats.Rats were intragastrically administered different doses of EGb761(50,100,and 200 mg·kg-1) on the 12th day after injection of FCA,the drugs were given for 16 days.Tripterygium wilfordii ployglycosidium(TPT) at 40 mg·kg-1 was used as a positive control drug.Rats were killed after FCA was injected on the 28th days.The activities of myeloperoxidase(MPO),superoxide dismutase(SOD),inducible nitric oxide synthase(iNOS),and the contents of malonyldialdehyde(MDA) and nitric oxide(NO) in serum were detected by biochemistry methods.The contents of TNF-α in serum were measured by enzyme-linked immunosorbent assay methods.RESULTS The secondary inflammation of AA rats appeared on the 12th day after injection of FCA.At the same time(d 12),different doses EGb761 were given to AA rats.It was found that EGb761 could significantly inhibit the secondary paw swelling of AA rats from the 20th day.Compared with the model group,the activities of MPO and iNOS and the contents of MDA,NO and TNF-α in serum were significantly decreased(P0.05 or P0.01);the activities of SOD in serum were increased(P0.05 or P0.01).CONCLUSION EGb761 can alleviate the inflammatory reactions in AA through inhibiting oxygen free reaction,exerting antioxidation effects and decreasing the production of TNF-α.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
Access and Citation
Cited By Count: 1
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot