Title: Effects of Polyporus Decoction on the Expression of AQP2 in Adriamycin-induced Nephropathy Rats
Abstract: Objective:To study the effects of Polyporus Decoction on the Expression of AQP2 in Adriamycin-induced Nephropathy Rats.Methods:110 SPF male SD rats were randomly divided into control group,sham operation group,model group,14 days after tail vein injection of adriamycin,Urine protein quantitation ≥ 100 g /24 prompt model copied successfully,The survival model group rats were again randomly divided into the model group,Polyporus Decoction group,furosemidegroup,Respectively gavage two weeks,four weeks,six weeks,eight weeks,some rats were sacrificed and preserve specimens of blood urine and kidney,Semi automatic biochemical analyzer detects urinary protein content,Liver and kidney function,etc,HE staining was applied to abserve renal pathological changes,the RT-PCR assay detect AQP2 mRNA expression,AQP2 protein expression was detected by Western Blot.Results:(1) Biochemical indicators:In gavage 8 weeks,Polyporus Decoction group average urine output is higher than the model group(P 0.05).Other biochemical parameters,no significant difference between the model group.(2) AQP2 mRNA:14 days after injection,the average expression level of AQP2 mRNA in model group were higher than control group,with a statistically significant difference(P 0.05),In gavage 2,4,6,8 weeks,the average expression level of AQP2 mRNA in Polyporus Decoction group were lower than the model group(P 0.05).(3) AQP2 protein:14 days after injection,the average expression level of AQP2 protein in model group were higher than control group,with a statistically significant difference(P 0.05),In gavage 4,6,8 weeks,the average expression level of AQP2 protein in Polyporus Decoction group were lower than the model group(P 0.05).Conclusion:The diuretic effect of Polyporus Decoction may be achieved by down-regulating the expression of AQP2 mRNA and AQP2 protein.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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