Title: Genetic diversity and differentiation of Chimonanthus nitens complex populations
Abstract: Genetic diversity and genetic relationship of 201 individuals from seven populations of Chimonanthus nitens complex including C.nitens,C.zhejiangensis and C.grammatus,were investigated with ISSR and RAPD markers.The result showed that:1) 12 out of 38 ISSR markers and 12 out of 80 RAPD markers were detected to be polymorphic.2) 142 and 226 bands were amplified with ISSR primers and RAPD primers,respectively,with PPL (percentage of polymorphic loci) values 94.37% and 95.13%.3) The mean Nei's gene diversity index (h) of populations using combined ISSR+RAPD data was 0.295 2 and Shannon's information index (I) was 0.488 4 which indicated a high level of genetic diversity.4) AMOVA analysis of genetic diversity showed that most of the variations exist within the populations.5) The dendrogram using UPGMA method clustered the seven populations into three groups,reflecting the relationship between geographical substructure and genetic diversity.The results provide forceful evidence for resolving the problem of ascription of C.zhejiangensis and C.grammatus at DNA level.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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Cited By Count: 1
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