Title: Protective effect of compound Ginkgo biloba on alcohol-induced liver injury and its mechanism
Abstract: OBJECTIVE To observe the protective effect of compound Ginkgo biloba(CGB) against alcohol-mediated hepatic injury and its mechanism.METHODS Rats were randomized into seven groups: normal control,model,Ginkgo biloba extract(GBE) 0.8 g·kg-1,CGB 2.4,0.8 and 0.4 g·kg-1,bifendate(Bif) 0.15 g·kg-1 groups.The alcohol-injured hepatic injury model was established after rats were ig given liquor-corn oil suspension for 10 weeks.And CGB,GBE and Bif were ig given in the meantime except normal and model control groups.The content of alamine aminotransferase(ALT) and aspartate aminotransferase(AST) in serum and that of malondialdehyde(MDA),superoxide dismutase(SOD),glutathione(GSH) in liver homogenate were measured,so were the hepatic histological and electron microscopy changes in liver mitochondria.Plasma concentrations of chlorzoxazone and DDS were tested before and after CGB was given by the method of cocktail,and their pharmacokinetic parameters were calculated to evaluate metabolism activities of cytochrome P-4502E1,CYP2E1 and CYP3A4 indirectly.RESULTS Liver cells were severely damaged by alcohol,resulting in alcoholic hepatitis and the fat liver.Compared with normal control group,the activities of ALT,AST and MDA increased(P0.01) while GSH and SOD decreased(P0.01).Compared with model group,ALT,AST and MDA obviously decreased(P0.05,P0.01) in CGB 2.4 and 0.8 g·kg-1 groups,while GSH and SOD were significantly increased(P0.05,P0.01).AUC0-24 and cmax for chlorzoxazone and DDS after CGB was given significantly decreased(P0.05,P0.01) compared to those before CGB was given ig in model groups.CONCLUSION CGB may protect the liver from alcoholic injury and the mechanism may be related to antioxidation.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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Cited By Count: 2
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