Title: Expression Activity of Cancer-Specific Adenovirus Vector Targeting Telomerase
Abstract: Objective To construct an adenovirus vector specifically targeting telomerase-positive cancers,and to investigate its capability of targeting transgene expression in cancer cells.Methods hTERT promoter was cloned into the multiple cloning sites of pDC312 plasmid to produce a transgene expression cassette.An insulator was inserted respectively into upstream and downstream of the cassette,then the adenovirus vector targeting telomerase-positive cancers was generated as pSG-TPE.The luciferase assay was applied to analyze the hTERT promoter activity.pSG-TPE carrying green fluorescent protein(EGFP) was used to recombine adenovirus AdTPE-EGFP.The restricted expression of EGFP mediated by AdTPE-EGFP was examined and compared with the control adenovirus AdCMV-EGFP regulated under the CMV promoter.Results hTERT promoter had no activity in normal cells,but nearly had as strong activity as SV40 promoter in cancer cells.AdTPE-EGFP could express EGFP specifically and steadily in cancer cells but not in normal cells,whereas AdCMV-EGFP could express EGFP both in cancer and normal cells.Conclusion The adenovirus vector pSG-TPE has a specificity of targeting transgene expression,with decreased toxicity to normal cells.The application of insulator in adenovirus vector interdicts the interference between the transgene expression cassette and the regulatory sequence of viral gene,and further increases the efficiency of transgene expression.The application of adenovirus vector pSG-TPE is a promising approach to the targeting gene therapy of tumor.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
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Cited By Count: 1
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