Title: Screening of stalk cellulose-degrading microorganisms and construction of a microbial community with high capacity
Abstract: 【Objective】 The cellulose-degrading bacteria were expected to be obtained from the soil,and get them mixed and cultured,then the microbial community degradating cellulose efficiently can be obtained to provide a theorotical basis on mixed fermentation between microorganisms.【Method】 Cellulose congo-red culture medium was used to screen soil samples,and then CMC,FPA,C1 and β-Gase were used to re-screen,by which the strains with high capacity were obtained.The stains were miyed cultured,and a microbial community with high capacity was screened.The strains were judged by the characteristics of the colony form and individual form.【Result】 Five strains that could decompose cellulose better were obtained from soil samples.Then they were cultured by mixed fermentation.The combination of ye-9/er-72/se-93 enhanced cellulase activities containing CMC,FPA,C1 and β-Gase to some degree,and the activity of CMC,FPA,C1 and β-Gase was 3.18,1.67,1.08 and 1.12 U/mL.The strains of ye-9,er-72 and se-93 were judged as actinomycetes preliminarily.【Conclution】 The study found that the enzyme activity of the microbial community was higher than that from the fermentation of single bacteria.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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Cited By Count: 1
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