Title: Inhibition of procyanidin from Vaccinium vitisidaea on proliferation of cardiac fibroblast in neonatal rats and its mechanism
Abstract: Objective To investigate the effect of procyanidin from Vaccinium vitisidaea on the proli-feration of cardiac fibroblast(CFb) induced by angiotensin Ⅱ(AngⅡ),and to explore its mechanism.Methods The CFb proliferation of cultured neonatal rat was induced by AngⅡ and detected by MTT assay.The levels of collagen Ⅰ,collagen Ⅲ,and TGF-β1 were measured by ELISA.The change of NO content and iNOS activity were measured by nitric acid reductase method and spectrophotometry.Cell cycle was assessed via flow cytometry(FCM). The expression of cell cycle regulatory protein p27 was determined by the combination of immunocytochemical staining and image analysis software.Results CFb Proliferation,collagen content,and TGF-β1 levels in culture medium were markedly inhibited when CFb were treated with procyanidin at concentration of 25,50,and 100 mg/L(P0.05 and 0.01).And iNOS-NO system activities in CFb were incerased(P0.05 and 0.01).In the FCM analysis,it was found that procyanidin could block CFb in the G0/G1 phase from entering the S phase,resulting in more cells in the G0/G1 phase and fewer in the S phase.The percentage of the cells in the G0/G1 phase and the S phase at the dosage of 25,50,and 100 mg/L were significantly different in comparison to the model group(P0.05 and 0.01).Procyanidin induced p27 expression with statistic significance compared to model group(P0.01).Conclusion The inhibition of procyanidin on CFb proliferation might be due to the stimulation of p27 expression and arresting of cells in G0/G1 phase.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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