Title: Expression of protein P40 in E coli and primary purification
Abstract: To obtain purified P40 protein for the further study on its function.Sequence coding for protein P40 was amplified from whole p40 gene by PCR method and cloned into pGEX\|4tl vector for expression.Expressed protein induced by IPTG was then purified with glutathione agrose by means of affinity chromatography.Results showed that the coding sequence of a new gene we cloned previously could be expressed in E.coli with the form of fusion with GST and the fusion protein existed in inclusion bodies of the bacteria.
Publication Year: 2001
Publication Date: 2001-01-01
Language: en
Type: article
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Cited By Count: 1
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