Title: Effect of Croton Alkaloid(CA)on Cell Cycle Apoptosis and Bcl-2 Gene Expression in Human Osteosarcoma Cell
Abstract: Objective:To investigate the apoptosis in human osteosarcoma cells induced by CA,and the relationship between this apoptosis and expression of Bcl-2.Methods:MG63OS cells were treated with various concent rations of CA.Proliferation was determined by cell count in a cytometer visuture in the presence or Absence of CA.The expression of apoptosis regulated gene Bcl-2 were detected using immunohistochemical staining.Results:A time dependent and dose dependent cell growth inhibition was shown after exposure to CA.The cells treated with CA showed initially G2/M phase arrest,which was followed by apoptosis(Annexin-V-FITC PI).CA could reduce the expression of apoptosis regulated gene Bcl-2.Conclusion:CA is able to induce the apoptosis in human osteosar coma cells through the initiation of G2/M phase arrest and inhibiting mitosis in both a time dependent and dose dependent manner.This apoptosis maybe mediated by down expression of apoptosis regulated gene Bcl-2.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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