Title: Extraction Technique and Content Determination of Total Flavonoids From the Leaves of Azadirachta indica
Abstract: Objective: To optimize extraction technique of total flavonoids and establish a method for measuring the contents of total flavonoids in the leaves of Azadirachta indica.Methods:The optimum process was investigated by L9(34) orthogonal design.The contents of total flavonoids in the leaves of Azadirachta indica were measured using spectrophotometry with a detection wavelength of 500nm,borrowing the Rutin as a contrast.Results:The optimum technical condition: 80% ethanol,refluxing and extracting for 3 times,one hour each time.The linear range was between 0.020~0.080mg/mL(R=0.9992) and the average recovery was 96.67% with corresponding RSD of 0.96%.Conclusion:The optimum extraction technology of total flavonoids is stable.The method for determination is simple,rapid and is suitable for determination of total flavonoids in the leaves of Azadirachta indicant.
Publication Year: 2009
Publication Date: 2009-01-01
Language: en
Type: article
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