Title: Determination of Tanshinone II_A in Radix Et Rhizoma Salviae Miltiorrhizae Microemulsion by HPLC
Abstract: Objective:To establish the determination method of Tanshinone Ⅱ_A in Radix Et Rhizoma Salviae Miltiorrhizae microemulsion by HPLC.Methods:The analysis was performed on a Diamonsil-C_(18) column(4.6 mm×250 mm,5 μm),with a mobile phase of methanol-water(80∶20).The flow rate was 1 mL·min~(-1).The detection wavelength was 270 nm.The column temperature was 35 ℃.Results: The calibration curve was linear in the range of(7.59~303.44)ng,r=(1.000 0).The accuracy,repeatability,stability and recovery of this method were satisfied.Conclusions:The established method of determinating for the content of Tanshinone Ⅱ_A in Radix Et Rhizoma Salviae Miltiorrhizae microemulsion was convenient,accurate and reliable.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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