Title: Functional Analysis on the Recombinant of Human α-Fetoprotein Gene cis-Acting Elements
Abstract: Three human hepatoma cell lines (HuH-7 , huH- 1 /c1-2 and HLE) and one human lung
adenocar- cinoma cell line were transfected with the six recombinant eukaryotic expression
vectors containing luciferase reporter gene under the control of six human α-fetoprotein
transcription-regulatory se- quences (AFP TRSs) . The results showed that the expression of
luciferase reporter gene was limited in AFP-producing hepatoma cells , and was associated with
the amount ofAFP production . Of the six recombinant AFP TRSS , the 2.2kb AFP TRS without
AFP Silencers could drive the luciferase gene expression most efficiently . On the other hand,
no luciferase gene expression was found in either AFP-nonproducing hepatoma cells or
nonhepatoma cells . Thus , hepatoma-specific AFP TRSs were achieved , which can be used to
direct the suicide gene expression in our further hepatoma gene therapy project.
Publication Year: 1999
Publication Date: 1999-01-01
Language: en
Type: article
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