Title: Activation of vascular endothelial cells improves the homing of hematopoietic stem cells
Abstract: AIM: To study the effect of endothelial cell activation on the homing of hematopoietic stem cells (HUHSC) during transplantation. METHODS: Human umbilical vein endothelial cells (HUVEC) were cultured to single layer and activated by vascular endothelial growth factor (EVGF),granulocyte colony stimulating factor (G-CSF) and lipopolysaccharide (LPS),respectively. The HUHSC,enriched by eliminating red blood cells,granulocytes,monocytes and lymphocytes from cord blood,were cocultured with activated HUVEC to make adhesion. The adhesive ability of activated HUVEC to C-Kit + HUHSC was assayed by ELISA. Anti-VCAM-1 monoclonal antibody was used to detect the effect of activation on HUVEC and HUHSC interactions. RESULTS: Resting HUVEC had a little adhesive ability to HUHSC. A great enhancement of adhesive ability was showed when HUVEC was activated by VEGF,G-CSF and LPS. In the presence of anti-VCAM-1,the adhesive ability of activated HUVEC was decreased remarkablely. CONCLUSION: HUHSC homing may be related to the activation of endothelial cells and adhesion molecules.
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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