Title: Evaluation on correlation between serum anti-nucleosome antibody and activity of systemic lupus erythematosus
Abstract: Objective To study the correlation between anti-nucleosome antibody(ANuA) and the activity of systemic lupus erythematosus(SLE),and the sensitivity and specificity of ANuA in SLE diagnosis.Methods 58 SLE patients were collected and divided into three groups according to SLE disease activity index(DAI),inactive group,mild activity group,moderate and severe activity group.Other 25 cases including other rheumatic diseases and health subjects were collected as the control group.Then ANA,ANuA,ds-DNA antibody,anti-Sm,C3 and ESR were detected by using indirect immunefluorescence,Western blotting,rate nephelometry assay and automatic ESR analyzers,etc.Results(1) The positive rate of ANuA was positively correlated with the disease activity level of SLE(r=0.638,χ2=40.235,P=0.00).(2) The sensitivity of ANuA in diagnosing SLE was 51.72%,which was higher than 20.69% of ds-DNA antibody and 41.38% of anti-Sm,the specificity of ANuA was 92%,which was close to 96% of ds-DNA antibody and 92% of anti-Sm.3.The ANuA expression level in SLE′ s serum was negatively correlated with C3(r=-0.595,P=0.00),and positively correlated with ESR(r=-0.783,P= 0.00),but had no significant correlation with ANA karyotype and titer(P0.05).Conclusion The ANuA detection is regarded as a new laboratory evaluation index with the close correlation with SLE activity,high sensitivity and strong specificity in diagnosing SLE,and is easy to operate,which will play an important role in SLE diagnosis,rational therapy and curative effects assessment.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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