Abstract: Event Abstract Back to Event Silk protein as a cell additive You-Young Jo1, Haeyong Kweon1 and Kwang-Gill Lee1 1 National Academy of Agricultureal Science, Department of Agricultural BIology, Korea Introduction: A substance for the cell proliferation, additives such as cell cytokine is a very expensive. It is known that silk protein supports effectively cell proliferation[1]-[4]. Although there are many varieties of Bombyx mori silkworm, the effect of silkworm varieties on cell proliferation has not been considered in detail. To develop the cell additives using silk proteins, we studied that effects on cell proliferation and characteristics of silk obtained from Korea various silkworm varieties. Materials and Methods: Silk sericin was prepared under high temperature high pressure condition. Silk fibroin was prepared using CaCl2 : H2O : ethanol (1:8:2) with different dissolution time(1, 3, 5 hour). The characteristics of each silk protein were analyzed by performing Tensile strength, amino acid composition, porosity, XRD and gel electrophoresis. We investigated the attachment, proliferation, morphology of the cells and the expression levels of genes related to cell growth. Results: There are differences in silk cocoon from different silkworm varieties. The porosity of cocoons was 73~85%. The crystal structure of each cocoon was different by XRD analysis[5]. Also amino acid composition was different. But the molecular weight of silk proteins was same. The proliferation was accelerated in the presence all of silk sericin and silk fibroin. Especially, this was the best Baekokjam silk sericin in cell proliferation. The Baekokjam silk serin was upregulated the EGF gene expression up to 5 times. Conclusion: We expect that silk proteins could be a preferable culture medium supplement for stimulating the proliferation of cell. Then, these results suggest silk as a new material for medium supplement. This study was carried out with the support of "Research Program for Agricultural Science & Technology Development (Project No. PJ010006)", National Academy of Agricultural Science, Rural Development Administration, Republic of KoreaReferences:[1] Aramwit P, Kanokpanont S, De-Eknamkul W, Kamei K, Srichana T (2009) The effect of sericin with variable amino-acid content from different silk strains on the production of collagen and nitric oxide. Journal of Biomaterials Science-Polymer Edition 20 1295~1306.[2] Bisceglie V (1933) Uber die antineoplasticsche immunitat: heterologe Einplflanzung von tumoren in Huhner-embryonen. Ztschr. Krebsforsch 40 122~140.[3] Cha HM, Kim SM, Choi YS (2015) Serum-free media supplement from silkworm gland for the expansion of mesenchymal stem cells. Tissue Engineering and Regenerative Medicine 12, 53~59.[4] Chen F, David P, Vollrath F (2012) Morphology and structure of silkworm cocoons. Materials Science and Engineering C 32, 772~778.[5] Um IC, Kwon HY, Lee KG, Park YH (2003) The role of formic acid in solution stability and crystallization of silk protein polymer. International Journal of Biological Macromolecules 33, 203~213. Keywords: Cell Proliferation, Gene Expression, Biocompatibility Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Cellular migration and biomaterials Citation: Jo Y, Kweon H and Lee K (2016). Silk protein as a cell additive. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00401 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers You-Young Jo Haeyong Kweon Kwang-Gill Lee Google You-Young Jo Haeyong Kweon Kwang-Gill Lee Google Scholar You-Young Jo Haeyong Kweon Kwang-Gill Lee PubMed You-Young Jo Haeyong Kweon Kwang-Gill Lee Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.