Title: Ginger leaf-induced reduction of cell viability is mediated by activating transcription factor 3 in human colorectal cancer cells
Abstract:W performed in vitro study to evaluate anti-cancer properties of ginger leaf (GL) and then elucidate the potential mechanisms. Exposure of GL to human colorectal cancer cells(HCT116, SW480 and LoVo ce...W performed in vitro study to evaluate anti-cancer properties of ginger leaf (GL) and then elucidate the potential mechanisms. Exposure of GL to human colorectal cancer cells(HCT116, SW480 and LoVo cells ) reduced the cell viability in dose-dependent manner. GL increased activating transcription factor 3 (ATF3) expression and activated ATF3 promoter activity, indicating transcriptional activation of ATF3 gene by GL. In addition, our data showed that GL-responsible sites might be between -514 and -85 region of the ATF3 promoter. We also observed that ERK1/2 inhibition by PD98059 attenuated GL-mediated ATF3 expression but not p38 inhibition by SB203580, indicating ERK1/2 pathway implicated in GLinduced ATF3 activation. These findings suggest that the reduction of cell viability and apoptosis by GL may be a result of ATF3 promoter activation and subsequent increase of ATF3 expression through ERK1/2 activation in human colorectal cancer cells.Read More
Publication Year: 2014
Publication Date: 2014-09-22
Language: en
Type: article
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