Title: Inhibition of asparagine-linked glycosylation by incorporation of a threonine analog into nascent peptide chains.
Abstract: he importance of threonine in the Asn-X-Thr recognition sequence for asparagine-linked glycosylation was tested by examining the effect of a threonine analog, 8-hydroxynorvaline, on co-translational glycosylation in Krebs' II ascites tumor lysates.8-Hydroxynorvaline inhibited the glycosylation of the a subunit of human chorionic gonadotropin and the 8 subunit of bovine luteinizing hormone; both proteins contain Asn-X-Thr recognition sites."he effect was prevented by threonine, indicating that 8-hydroxynorvaline acted via its incorporation into protein.These results provide direct evidence for the role of threonine in Asn-X-Thr sites for asparagine-linked glycosylation.The results support the view that this site is sensitive to steric hindrance.