Title: Solid state fermentation condition optimizing of Trichoderma viride C-08 for producing cellulase.
Abstract: Trichoderma viride C-08 can very efficiently produce cellulase and xylanase.The optimal conditions of the solid-state fermentation for Trichoderma viride to produce cellulase and xylanase were studied.The results showed that optimal solid culture medium had ratios of straw to bran 2:4.NH4+ source was good for C-08 to produce cellulose and NO3-source was good for C-08 to produce xylanase,so NH4NO3 source was the best.The ratio of stuff to water 1:2 was good for C-08 CMCase production,and 1:2.5 for Xylanase production.The optimal rice straw granularity 150-425μm was suitable for C-08 CMCase production.Granularity below 150μm and above 425μm was best for xylanase production.The optimal pH for C-08 fermentation was 4.The optimal conditions for cellulase and xylanase production were as follows: inoculum size 7.5×107,incubation temperature lower than 28℃,fermentation period 72h.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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