Abstract: As a novel self-assembly method developed in recent years, DNA origami is one of the greatest progress in the field of DNA nanotechnology and DNA self-assembly. Different from traditional DNA self-assembly, DNA origami, on the basis of the hybridization of a long circular genomic single stranded DNA with a group of staple strands, can be used to construct two- or three- dimensional sophisticated shapes at the nanoscale. Moreover, the nanostructures by DNA origami are more predictable, precise, controllable and efficient. The merits also include relatively low requirements for the experimental conditions and operation skills. A variety of functional nanoparticles can be assembled onto the DNA origami nanoscaffolds, to obtain complicate nanodevices with special functions. Therefore, DNA origami has shown great potential in nanotechnology. This review describes the progress of DNA origami in the assembly of diverse DNA nanostructures and the prospect of DNA origami in future.