Abstract: We have studied on the determination of dehydroacetic acid (DHA) in foodstuffs, after pretreated by solvent extraction method, distillation method or steam distillation method.The results obtained were as follows:1. The interfering substances were found a little by steam distillation method but better result was obtained than the other methods.2. The separation of DHA by steam distillation, was carried out after the sample solution was saturated with sodium chloride and then tartaric acid. was added The distillate was determined spectrophotometrically at 290mμ. This method was applied to market foods, for example, instant orange juice, starch, butter and cheese.