Title: Inhibition of Growth and Sclerotium Formation in <i>Rhizoctonia Solani</i> by Garlic Oil
Abstract: Oil of garlic (Allium sativum L.) in natural and synthetic forms has been reported to suppress the activity of many air and soil-borne fungi (Murthy and Amonkar, 1974). Several other workers have observed the antimicrobial activity of extracts of garlic (Agrawal, 1978; Fliermans, 1973; Rao et al., 1947; Tansey and Apleton, 1975). Singh et al. (1979) have recently shown that even garlic-leaf extract significantly reduced the growth of Sclerotinia sclerotiorum (Lib.) deBary and Fusarium oxysporum f. sp. ciceri Padwick causing wilts in gram (Cicer arietinum L.) We report here the inhibition of growth and sclerotium formation in Rhizoctonia solani Kuhn, incitant of root rot of gram, by garlic oil. Rhizoctonia solani was isolated from infected roots of gram and grown on potato-dextrose-agar (peeled potato 250 g, dextrose 20 g, distilled water 1 liter) slants. Index of antifungal effect of garlic oil on growth of the pathogen was obtained by measuring the growth in the form of dry weight in SP3 liquid medium (glucose 50 g, yeast extract 1 g, peptone 1 g, KH2PO4 1.5 g, (NH4)2SO4 1.5 g, MgSO4 1 g, agar 20 g, distilled water 1,000 ml). A 25-ml aliquot of the medium was poured in 150-ml flasks containing 50, 100, 150, 200 and 250 ppm of sterilized oil. Garlic oil was sterilized by passing through a millipore filter (0.22 gm) using a Swinny filter adapter. Each flask was inoculated with a 2-mm mycelial disc of the fungus. Control flasks were inoculated with equal-sized mycelial discs, but without garlic oil. The flasks were incubated at 25 ± 2 C for 21 da. Growth was recorded after 7, 14 and 21 da by harvesting through filtration on oven-dried Whatman filter paper No. 42. The filters containing fungal growth were
Publication Year: 1980
Publication Date: 1980-09-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 16
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