Title: The decolorization of azo dyes by <i>Trametes versicolor</i> under the influence of different glucose concentrations
Abstract: Various azo-dyes can be found in environment as a result of textile industry activities and most of them are harmful for human and animal health. The decolorization and removal of most of these compounds usually requires complex physico-chemical treatment of the wastewaters and is not quite effective. The following biological treatment where the main components are microorganisms becomes still more important. The white rot fungi are distinguished among most of the microbial strains discussed in the literature for its ability to decolorize various industrially applied dyes. A white rot fungi Trametes versicolor strain capable of decolorizing various textile dyes was investigated in this study. The strain identification was performed on the basis of 18S rDNA analysis (Acc No GQ472774). The decolorization of two widely used azo dyes (Orange II, Congo Red) was followed in conditions of mineral medium cultivation with different concentrations of dye (50mg/l and 125mg/l) and glucose (1, 2 and 3%) included in a medium. The strain ability to decolorize listed azo dyes was established by UV–Vis spectroscopy of samples taken before and at every 24 hours during dye decolorization in the culture medium. The processes of decolorization were performed under aerobic condition at pH 6.5 and 28 °C. The data obtained gave proof of the fact that higher glucose concentration actually lowered the specific decolorization rate. Nevertheless, the biggest percent of decolorization observed in a medium containing 3% glucose is due to the longer time of growth and development of microbial culture and respectively longer laccase production. The observed laccase (EC 1.10.3.2) activity dynamics indicated the involvement of this enzyme in the decolorization processes. The best results were obtained in a cultivation medium complemented with 3% glucose in which the strain Trametes versicolor 1 decolorized 125 mg/l of each investigated dye as follow: 90% of Orange II within 336 h and 90 % of Congo Red within 360 h.
Publication Year: 2010
Publication Date: 2010-12-01
Language: en
Type: article
Indexed In: ['crossref']
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