Publication Information

{'id': 'https://openalex.org/W2324384628', 'doi': 'https://doi.org/10.1021/jf202746a', 'title': 'Gene Cloning, Expression, and Characterization of a Nitrilase from <i>Alcaligenes faecalis</i> ZJUTB10', 'display_name': 'Gene Cloning, Expression, and Characterization of a Nitrilase from <i>Alcaligenes faecalis</i> ZJUTB10', 'publication_year': 2011, 'publication_date': '2011-09-13', 'ids': {'openalex': 'https://openalex.org/W2324384628', 'doi': 'https://doi.org/10.1021/jf202746a', 'mag': '2324384628', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/21913706'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1021/jf202746a', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S134644764', 'display_name': 'Journal of Agricultural and Food Chemistry', 'issn_l': '0021-8561', 'issn': ['0021-8561', '1520-5118'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320006', 'host_organization_name': 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112, 136], 'verified': [42], 'by': [43], 'site-directed': [44], 'mutagenesis,': [45], 'detailed': [48], 'mechanism': [49], 'this': [51, 149], 'clarified.': [54], 'The': [55, 72, 103], 'substrate': [56], 'specificity': [57], 'study': [58], 'demonstrated': [59], 'belongs': [66], 'to': [67], 'family': [69], 'arylacetonitrilases.': [71], 'optimum': [73], 'pH': [74], 'temperature': [76], 'for': [77, 107], 'purified': [79], '7–8': [82], '40': [84], '°C,': [85], 'respectively.': [86, 118], 'Mg2+': [87], 'stimulated': [88], 'hydrolytic': [89], 'activity,': [90], 'whereas': [91], 'Cu2+,': [92], 'Co2+,': [93], 'Ni2+,': [94], 'Ag+,': [95], 'Hg2+': [97], 'showed': [98], 'strong': [100], 'inhibitory': [101], 'effect.': [102], 'Km': [104], 'vmax': [106], 'mandelonitrile': [108, 126], 'were': [109], '4.74': [110], 'mM': [111, 132], '15.85': [113], 'μmol': [114], 'min–1': [115], 'mg–1': [116], 'protein,': [117], 'After': [119], '30': [120], 'min': [121], 'reaction': [122], 'using': [123], 'nitrilase,': [125], 'at': [127], 'concentration': [129], '20': [131], 'completely': [134], 'hydrolyzed': [135], 'enantiomeric': [138], 'excess': [139], 'against': [140], '(R)-(−)-mandelic': [141], 'acid': [142], '>99%.': [144], 'Characteristics': [145], 'investigation': [146], 'indicates': [147], 'is': [151], 'promising': [152], 'in': [153], 'catalysis': [154], 'applications.': [155]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2324384628', 'counts_by_year': [{'year': 2023, 'cited_by_count': 2}, {'year': 2022, 'cited_by_count': 3}, {'year': 2021, 'cited_by_count': 1}, {'year': 2020, 'cited_by_count': 4}, {'year': 2019, 'cited_by_count': 2}, {'year': 2018, 'cited_by_count': 2}, {'year': 2017, 'cited_by_count': 5}, {'year': 2016, 'cited_by_count': 4}, {'year': 2015, 'cited_by_count': 7}, {'year': 2014, 'cited_by_count': 7}, {'year': 2013, 'cited_by_count': 7}, {'year': 2012, 'cited_by_count': 3}], 'updated_date': '2024-12-19T01:45:22.469541', 'created_date': '2016-06-24'}