Title: Molecular Markers Application for Blast Resistance Selection on the Double Haploid Rice Population
Abstract: Blast disease, caused by fungal Pyricularia grisea Sacc., is one of the most devastating diseases in rice. The use of blast-resistant rice varieties is one of the most e fficient ways to control blast disease in rice. Bla st-resistant varieties can be produced through breeding. The use of marker-assisted selection (MAS) available to support selectio n of resistant lines based on resistance gene. The objective of th is research was to compare the resistance response of the double haploid lines with the differential varieties to th ree selected Indonesian blast races and to identify the resistance genes caused the resistance to blast based on the resista nce response and the genotype evaluation using molecular markers. Forty-nine double haploid lines from a double cross ing IR54/Parekaligolara//Bio110/Markuti were selected using molecular markers based on the targeted genes Pib , Pi1 , Pi2 , Pi9 , Pi33 , Pir4 , and Pir7 . To compare the phenotype selection, ten LTH monogenic lines of differential varieties were used. All plants tested were inocula ted by three selected Indonesian blast races. The results show t hat the Pib gene caused a resistance to race 123, while the Pi1 and Pir7 genes caused a resistance to race 123 and 133. The Pi2 , Pi9 , Pi33 , and Pir4 genes did not cause a resistance to race 123, 133, or 173.
Publication Year: 2014
Publication Date: 2014-06-03
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 4
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