Title: Growth and Differentiation of Daphnia Mana Es in vitro.
Abstract: In the course of some ultra-centrifuging experiments there arose the need of rearing the parthenogenetic eggs of Cladocera in vitro. A review of the literature revealed no record of the successful growth of eggs of this group of animals outside the brood-chamber of the mother. We have succeeded in rearing the eggs of Daphnia magna in tissue culture slides and inasmuch as the embryos are transparent, this enabled us to make observations on some of the sequence of events in the course of the development of the embryos without the necessity of staining and sectioning.A number of different media were used in an endeavor to grow Daphnia magna eggs in vitro. A modified Ringer's solution utilized successfully for physiological work on isolated organs of Cladocera by Levy,1 proved injurious to the eggs. The growth of eggs in a medium consisting of the fluid obtained from the brood-chamber of animals in which there were developing eggs showed no advantage over a simpler medium consisting of sterile pond water. Thi...
Publication Year: 1940
Publication Date: 1940-03-01
Language: en
Type: article
Indexed In: ['crossref']
Access and Citation
Cited By Count: 6
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