Title: Thermal Optimization of α-amylase Production in Brevibacillus sp
Abstract: Thermostability is a desired characteristic of most of the industrial enzymes. The production of ?-amylases has been thoroughly investigated and observed that it was affected by a variety of physiochemical factors, such as the composition of the growth medium under various temperature parameters with constant pH 7.0. The enzyme production was assayed in submerged fermentation (SmF) condition. The optimum production of ?-amylase by SmF was observed at 40C in Clarks and Lub Medium (2551 1 U/ml), Soybean Casein Digest Medium (920 0.13 U/ml) and Tryptone Glucose Beef Extract Medium (260 U/ml). The optimum production of ?-amylase by SmF was observed at 250C in Nutrient Broth (270 U/ml). The optimum production of ?-amylase by SmF was observed at 370C in Pikovskayas Medium (3371 0.50 U/ml), Tendler's Non-synthetic Medium (761 0.50 U/ml), Amylase Production Medium (580 U/ml) and Soluble Starch Beef Extract Medium (1930 U/ml). The optimum production of ?-amylase by SmF was observed at 500C in Luria Bertain Broth (240 0.12 U/ml) and Yeast extract peptone Glycerol Glucose Medium (265 U/ml). Thermal variability of ?-amylase produced by Brevibacillus borostelensis R1 in various media may have a significant role in many applications like bakery industry, treatment of effluents from sago and rice industry, sewage water treatment, fodder production, laundry industry and textile industry.