Title: Immunohistochemical detection of the nm23-H1 protein in gallbladder and biliary tract tumours
Abstract: Nm23-H1 protein immunoreactivity was studied in gallbladder (n = 13), common bile duct (n = 5) and ampulla tumours (n = 7) together with chronic cholecystitis (n = 11) and preneoplastic lesions of the gallbladder (n = 4) and ampulla (n = 3), using the monoclonal antibody, NM 301 (Molecular Oncology Inc.). Absent or weak nm23-H1 immunostaining was found in most (67%) of the poorly differentiated gallbladder adenocarcinomas and in half of the moderately differentiated adenocarcinomas. The difference in nm23-H1 protein immunostaining between gallbladder carcinoma and chronic cholecystitis was statistically significant (p = 0.0022; X2 test). Significant correlation was found between prolonged patient survival and nm23-H1 expression in gallbladder carcinomas (r = 0.652, p = 0.0l6, Spearman’s rank test). Most gallbladder dysplasias and adenoma had no reduction in nm23-H1 expression. All common bile duct carcinomas, most (67%) ampullary carcinomas in situ and some (43%) ampullary carcinomas had moderate to strong nm23-Hl immunostaining. In summary, low nm23-H1 protein immunoreactivity occured frequently in gallbladder carcinomas and this was associated with reduced patient survival. Differences in nm23-H1 protein immunoreactivity in common bile duct and ampullary carcinomas suggest that these tumours have a different molecular origin to gallbladder cancers. Nm23-H1 protein immunoreactivity was studied in gallbladder (n = 13), common bile duct (n = 5) and ampulla tumours (n = 7) together with chronic cholecystitis (n = 11) and preneoplastic lesions of the gallbladder (n = 4) and ampulla (n = 3), using the monoclonal antibody, NM 301 (Molecular Oncology Inc.). Absent or weak nm23-H1 immunostaining was found in most (67%) of the poorly differentiated gallbladder adenocarcinomas and in half of the moderately differentiated adenocarcinomas. The difference in nm23-H1 protein immunostaining between gallbladder carcinoma and chronic cholecystitis was statistically significant (p = 0.0022; X2 test). Significant correlation was found between prolonged patient survival and nm23-H1 expression in gallbladder carcinomas (r = 0.652, p = 0.0l6, Spearman’s rank test). Most gallbladder dysplasias and adenoma had no reduction in nm23-H1 expression. All common bile duct carcinomas, most (67%) ampullary carcinomas in situ and some (43%) ampullary carcinomas had moderate to strong nm23-Hl immunostaining. In summary, low nm23-H1 protein immunoreactivity occured frequently in gallbladder carcinomas and this was associated with reduced patient survival. Differences in nm23-H1 protein immunoreactivity in common bile duct and ampullary carcinomas suggest that these tumours have a different molecular origin to gallbladder cancers.
Publication Year: 1995
Publication Date: 1995-01-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 7
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