Title: On the Determination of Phosphates in Foods
Abstract:To establish the determination methods of phosphates in foods, the following methods were examined, being followed with satisfactory results. Polyphosphates contained in foods could be separated quali...To establish the determination methods of phosphates in foods, the following methods were examined, being followed with satisfactory results. Polyphosphates contained in foods could be separated qualitatively by thin-layer chromatography using Avicel-SF cellulose powder as adsorbent and a mixture of 0.5% pyridine-acetone solution and 20% trichloroacetic acid solution as the developing solvent. The quantity of phosphates was determined indirectly by determining the amount of molybdenum by atomic absorption spectrophotometry, after the formation of complex between phosphate and molybdate. Ion-exchange column chromatographic technique was applied for the separation of various kinds of polyphosphates contained in soft drink. These methods were applied to various kinds of foods, and from our results it was found that polyphosphates were present only in pressed ham, sausage and soft drink, and the amount of phosphates added to foods was found to be not so much.Read More