Title: An Estimation of Environmental Condition in Plant Tissue Culture and Growth of Callus Tissues Derived from Rice Embryos
Abstract: To inquire the effect of physical factors on cell growth and differentiation in plant tissue culture, an estimation was performed on environmental conditions in which our experiments were proceeding using petri dishes, Erlenmeyer flasks and test tubes.Besides on temperature conditions, characteristics in wavelength of monochromatic light produced by interference filters, prexiglass or colored-cellophane were measured spectro-radiometrically. Light intensity in petri dishes used was not essentially different from that at outside of dishes, but in Erlenmeyer flasks capped with aluminium foil, light energy reduced to 50 per cent in white light and below 3 per cent in monochromatic light at inside of the flasks. As illuminated monochromatic light obliquely, it was able to make the difference of light energy in and out of the flasks be a little.A conspicuous difference was not found between internal and external temperature of test tubes in glass rooms under natural light. Concerning effects of temperature condition on growth of rice callus tissues, a difference was observed of sensitivities to low temperature in rice varieties. At 10°C more remarkable decline was obtained in callus growth of Tan-ginbozu variety than of Aichi-asahi.